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processing raw airyscan data  (Oxford Instruments)


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    Structured Review

    Oxford Instruments processing raw airyscan data
    WT and variant channel-ER colocalization is similar in homo- and heterozygous conditions. (a) <t>Airyscan</t> microscopy images of RNCs transiently co-transfected with WT or variant flag-Na v 1.5 (green) and the ER marker calreticulin-DsRed2 (red), representing homologous conditions. Nuclei are stained with DAPI (blue). Right column: merged images. (b) Airyscan microscopy images of RNCs transiently co-transfected with GFP-Na v 1.5 WT (green), flag-Na v 1.5 WT or variants (not shown), and calreticulin-DsRed2 (red). Right column: merged images; nuclei are stained with DAPI (blue). (c) Quantification of Na v 1.5-ER colocalization in homozygous conditions. Colocalization is defined as percentage of Na v 1.5 signal area colocalizing with ER signal area within predefined ROI. (d) Quantification of colocalization of WT-Na v 1.5 with ER in the presence of variants, representing heterozygous conditions. Cell areas in which Na v 1.5 and ER colocalize are: WT, 57.4 ± 9.3%; Y87C, 54.0 ± 12.6%; R104W, 75.1 ± 9.5%; and R121W, 47.9 ± 10.5%. Scale bar, 10 µm. Data are presented as mean ± SEM. Groups were not significantly different.
    Processing Raw Airyscan Data, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 41025 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/processing raw airyscan data/product/Oxford Instruments
    Average 99 stars, based on 41025 article reviews
    processing raw airyscan data - by Bioz Stars, 2026-03
    99/100 stars

    Images

    1) Product Images from "Calmodulin binds to the N-terminal domain of the cardiac sodium channel Na v 1.5"

    Article Title: Calmodulin binds to the N-terminal domain of the cardiac sodium channel Na v 1.5

    Journal: Channels

    doi: 10.1080/19336950.2020.1805999

    WT and variant channel-ER colocalization is similar in homo- and heterozygous conditions. (a) Airyscan microscopy images of RNCs transiently co-transfected with WT or variant flag-Na v 1.5 (green) and the ER marker calreticulin-DsRed2 (red), representing homologous conditions. Nuclei are stained with DAPI (blue). Right column: merged images. (b) Airyscan microscopy images of RNCs transiently co-transfected with GFP-Na v 1.5 WT (green), flag-Na v 1.5 WT or variants (not shown), and calreticulin-DsRed2 (red). Right column: merged images; nuclei are stained with DAPI (blue). (c) Quantification of Na v 1.5-ER colocalization in homozygous conditions. Colocalization is defined as percentage of Na v 1.5 signal area colocalizing with ER signal area within predefined ROI. (d) Quantification of colocalization of WT-Na v 1.5 with ER in the presence of variants, representing heterozygous conditions. Cell areas in which Na v 1.5 and ER colocalize are: WT, 57.4 ± 9.3%; Y87C, 54.0 ± 12.6%; R104W, 75.1 ± 9.5%; and R121W, 47.9 ± 10.5%. Scale bar, 10 µm. Data are presented as mean ± SEM. Groups were not significantly different.
    Figure Legend Snippet: WT and variant channel-ER colocalization is similar in homo- and heterozygous conditions. (a) Airyscan microscopy images of RNCs transiently co-transfected with WT or variant flag-Na v 1.5 (green) and the ER marker calreticulin-DsRed2 (red), representing homologous conditions. Nuclei are stained with DAPI (blue). Right column: merged images. (b) Airyscan microscopy images of RNCs transiently co-transfected with GFP-Na v 1.5 WT (green), flag-Na v 1.5 WT or variants (not shown), and calreticulin-DsRed2 (red). Right column: merged images; nuclei are stained with DAPI (blue). (c) Quantification of Na v 1.5-ER colocalization in homozygous conditions. Colocalization is defined as percentage of Na v 1.5 signal area colocalizing with ER signal area within predefined ROI. (d) Quantification of colocalization of WT-Na v 1.5 with ER in the presence of variants, representing heterozygous conditions. Cell areas in which Na v 1.5 and ER colocalize are: WT, 57.4 ± 9.3%; Y87C, 54.0 ± 12.6%; R104W, 75.1 ± 9.5%; and R121W, 47.9 ± 10.5%. Scale bar, 10 µm. Data are presented as mean ± SEM. Groups were not significantly different.

    Techniques Used: Variant Assay, Microscopy, Transfection, Marker, Staining



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    processing raw airyscan data  (Oxford Instruments)
    99
    Oxford Instruments processing raw airyscan data
    WT and variant channel-ER colocalization is similar in homo- and heterozygous conditions. (a) <t>Airyscan</t> microscopy images of RNCs transiently co-transfected with WT or variant flag-Na v 1.5 (green) and the ER marker calreticulin-DsRed2 (red), representing homologous conditions. Nuclei are stained with DAPI (blue). Right column: merged images. (b) Airyscan microscopy images of RNCs transiently co-transfected with GFP-Na v 1.5 WT (green), flag-Na v 1.5 WT or variants (not shown), and calreticulin-DsRed2 (red). Right column: merged images; nuclei are stained with DAPI (blue). (c) Quantification of Na v 1.5-ER colocalization in homozygous conditions. Colocalization is defined as percentage of Na v 1.5 signal area colocalizing with ER signal area within predefined ROI. (d) Quantification of colocalization of WT-Na v 1.5 with ER in the presence of variants, representing heterozygous conditions. Cell areas in which Na v 1.5 and ER colocalize are: WT, 57.4 ± 9.3%; Y87C, 54.0 ± 12.6%; R104W, 75.1 ± 9.5%; and R121W, 47.9 ± 10.5%. Scale bar, 10 µm. Data are presented as mean ± SEM. Groups were not significantly different.
    Processing Raw Airyscan Data, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/processing raw airyscan data/product/Oxford Instruments
    Average 99 stars, based on 1 article reviews
    processing raw airyscan data - by Bioz Stars, 2026-03
    99/100 stars
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    WT and variant channel-ER colocalization is similar in homo- and heterozygous conditions. (a) Airyscan microscopy images of RNCs transiently co-transfected with WT or variant flag-Na v 1.5 (green) and the ER marker calreticulin-DsRed2 (red), representing homologous conditions. Nuclei are stained with DAPI (blue). Right column: merged images. (b) Airyscan microscopy images of RNCs transiently co-transfected with GFP-Na v 1.5 WT (green), flag-Na v 1.5 WT or variants (not shown), and calreticulin-DsRed2 (red). Right column: merged images; nuclei are stained with DAPI (blue). (c) Quantification of Na v 1.5-ER colocalization in homozygous conditions. Colocalization is defined as percentage of Na v 1.5 signal area colocalizing with ER signal area within predefined ROI. (d) Quantification of colocalization of WT-Na v 1.5 with ER in the presence of variants, representing heterozygous conditions. Cell areas in which Na v 1.5 and ER colocalize are: WT, 57.4 ± 9.3%; Y87C, 54.0 ± 12.6%; R104W, 75.1 ± 9.5%; and R121W, 47.9 ± 10.5%. Scale bar, 10 µm. Data are presented as mean ± SEM. Groups were not significantly different.

    Journal: Channels

    Article Title: Calmodulin binds to the N-terminal domain of the cardiac sodium channel Na v 1.5

    doi: 10.1080/19336950.2020.1805999

    Figure Lengend Snippet: WT and variant channel-ER colocalization is similar in homo- and heterozygous conditions. (a) Airyscan microscopy images of RNCs transiently co-transfected with WT or variant flag-Na v 1.5 (green) and the ER marker calreticulin-DsRed2 (red), representing homologous conditions. Nuclei are stained with DAPI (blue). Right column: merged images. (b) Airyscan microscopy images of RNCs transiently co-transfected with GFP-Na v 1.5 WT (green), flag-Na v 1.5 WT or variants (not shown), and calreticulin-DsRed2 (red). Right column: merged images; nuclei are stained with DAPI (blue). (c) Quantification of Na v 1.5-ER colocalization in homozygous conditions. Colocalization is defined as percentage of Na v 1.5 signal area colocalizing with ER signal area within predefined ROI. (d) Quantification of colocalization of WT-Na v 1.5 with ER in the presence of variants, representing heterozygous conditions. Cell areas in which Na v 1.5 and ER colocalize are: WT, 57.4 ± 9.3%; Y87C, 54.0 ± 12.6%; R104W, 75.1 ± 9.5%; and R121W, 47.9 ± 10.5%. Scale bar, 10 µm. Data are presented as mean ± SEM. Groups were not significantly different.

    Article Snippet: We used ZEN 2.1 software for processing raw Airyscan data, and the IMARIS coloc tool (IMARIS 9.3.1 software, Bitplane, Zürich, Switzerland) to quantify the colocalization between signals of two channels within the predefined ROI (region of interest, defined as the cell area).

    Techniques: Variant Assay, Microscopy, Transfection, Marker, Staining